If you look close at the reflection in the eye of the fish you will see three crosses on the hill of Calvalry depicting the crucifixion of Christ. There are 12 fish besides the Mackerel which represents the 12 apostles. The head of the fish on the far left of the painting goes off the edge of the canvas, this one represents James, the brother of Jesus who was said to have been beheaded. Judas is portrayed by the shark swimming just above the Mackerel between the two top fins. The grouping of four fish in the same pane of glass suggests Matthew, Mark, Luke, and John. The first four books of the Bible known as “The Gospels”.